Master of Laboratory Animal Science (FELASA-D), University of Copenhagen, Denmark- Year 2011-2014

B.V.Sc and A.H., Kerala Agricultural University, 2003.

PhD (ongoing) (2016 Dec onwards)- University of Copenhagen, Denmark.

Scientist F, Scientist in Charge, Laboratory Animal Science Division, BMT, SCTIMST, 2021- current position.

Scientist E and in Charge, Division of Laboratory Animal Science, BMT , SCTIMST, 2017-2021

Scientist-D DLAS, SCTIMST, BMT Wing, August 2014- 2017

Scientist-C DLAS, SCTIMST, BMT Wing, August 2010- August 2014

Veterinary Surgeon (Temporary) DLAS, SCTIMST, BMT Wing from September 2005 August 2006

Veterinary Surgeon DIMT, SCTIMST BMT Wing 11-12-2003 to September 2005

Research Associate College of Agriculture, Vellayani, May 2003 - September 2003

International Young Scientist Award 2009- Japanese Association for Laboratory Animal Science (https://www.jalas.jp/english/en_award.html)

International Best Poster Award- Dental burr assisted laminectomy in spinal cord injury rat model: Acute and chronic effects on welfare and outcome- Presenting Author- BEST POSTER AWARD at the 50th Anniversary Scand-LAS meeting; Tallinn, Estonia 2-4 November 2021.

PhD Grant to cover Tuition Fee- University of Copenhagen (2016)- 20 Lakhs (1,80,000 DKK)- In Vivo Pharmacology and Experimental Animals; The Department of Experimental Medicine.

Educational Award Bursary awarded for doing post graduation at University of Copenhagen, from Laboratory Animals, London, United Kingdom, 2010.

Lundbeck and Novo-Nordisk scholarship 2013 to perform residential Master's Project at Department of Veterinary Disease Biology, Section of Experimental Animal Models, University of Copenhagen, Denmark.

FELASA Travel Grant (partial scholarship) to present a Scientific Paper on Refinement of Spinal Cord Injury rat model at the FELASA Congress at Marseilles, France 2022 June

SGV- Swiss Scholarship 2013 to participate in Masters Project to complete FELASA category D programme at The University of Copenhagen, Denmark.

SCAND-LAS Scholarship to attend SCAND-LAS workshop 2013, at Tallinn, Estonia.

9th World Congress on Alternatives to Animal Experimentation- Invitational nomination and funding to participate as "session-co chair" in scientific session- Distress Evaluation in Rodents held at Prague, Czech Republic during August 2014.

First Rank Holder in Animal House Supervisors Training Programme Organized by NIN, NCLAS, (ICMR organization) Hyderabad, India September-November, 2007 in Breeding, Management, Care and Health Monitoring aspects.

FELASA-ICLAS-LAL competitive bursary and SCAND-LAS award to attend 13th FELASA congress, Brussels Belgium in 2016.

Laboratory animal science and medicine, Refinement of animal models, Welfare

Department of Veterinary and Animal Sciences, Section for Experimental Animal Models, Uni. of Copenhagen, Denmark

Department of Experimental Medicine, Uni. of Copenhagen, Section of Research and Education, Denmark

Breeding, Management, welfare assessments, Quality Control, statutory and regulatory compliance of small laboratory animals and the facility.

Convening and coordinating the activities advisory committee to animal experiments (ACAE) processing of documents to support animal experimentation of the Institute.

Working in animal models for hypertensive, orthopedic, neurological and wound healing portfolios.

Training programme in animal handling, welfare and ethics, CPCSEA regulations and laws in India, ethics and basic techniques- for Phd students and research scholars conducted by DLAS, BMT Wing, SCTIMST and also as a coursework for PhD's and M.Phil students.

Training to Laboratory Animal Veterinarians and Animal facility managers training course conducted by DLAS, BMT Wing, SCTIMST upon formal and periodic requests.

Life member (reg. no: 6062) in the Laboratory Animal Scientists Association (LASA), Registered Society under A.P society registration act, 2001. Registration number 415 of 2004.

Life Member of Laboratory Animal Scientists Association (LASAI), India.

Member of the IVA- Indian Veterinary Association.

Member of SCAND-LAS- Scandinavian Association of Laboratory Animal Science.

Member of ESLAV- European society of Laboratory Animal Veterinarians.

Member of SGV- Swiss Laboratory Animal Science Association.

Co-investigator and phase PI for proof of concept phase of two wound healing portfolio projects in TRC

Selected as a Member of CPCSEA, New Delhi 2021 for three years; published as per a notification in Gazette of India.

Dr. P.V. Mohanan, HOD, Division of Applied Biology

Santosh Kumar- Technical Assistant- A

Pradeep Kumar B, Animal Handler- B

Manoj M , Animal Handler- B

Sunil Kumar M- Animal Handler- B

Harikrishnan VS, Hansen AK, Abelson KS, Sorensen DB. A comparison of various methods of blood sampling in mice and rats: Effects on animal welfare. Laboratory animals. 2018 Jun;52(3):253-64.

Harikrishnan VS, Palekkodan H, Fasaludeen A, Krishnan LK, Abelson KS. Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon. 2021 Jul 1;7(7):e07500.

VS Harikrishnan, Lissy K. Krishnan, and Klas SP Abelson. "A novel technique to develop thoracic spinal laminectomy and a methodology to assess the functionality and welfare of the contusion spinal cord injury (SCI) rat model." PlOS ONE 14, no. 7 (2019): e0219001.

Harikrishnan, VS, Shenoy, Sachin J, Umashankar, PR, Anaesthetic regimen for coronary stenting in porcine model, Indian Veterinary Journal, 2006, 83; 486-489

Harikrishnan VS, Ranaraj VR, Fernandez AC. Incidence of Chirodiscoides caviae in laboratory rats-screening, identification and treatment. Scand J Lab Anim Sci. 2009;36(2):147?153. https://doi.org/10.23675/sjlas.v36i2.179

VS HARIKRISHNAN, SJ SHENOY, VR RANARAJ, S SUKHIJA, AK OLOYO A report on less-severe, long-duration persistent hind-limb ischemia surgical rabbit model Journal of the Hellenic Veterinary Medical Society; doi: 10.12681/jhvms.29395 Vol 72, No 4 (2021) A

Harikrishnan, VS, Shenoy, Sachin J, Umashankar, PR, Long-duration anesthesia for experimental surgical procedures in pig model, Indian Veterinary Journal, 2006, 83; 1173-1175

Harikrishnan VS, Umashankar PR. Cerebral protection for the preclinical evaluation of a vascular graft in sheep carotid artery model. Scandinavian Journal of Laboratory Animal Science. 2015 Jan 1;41(3):2002-0112. https://doi.org/10.23675/sjlas.v41i0.326

Harikrishnan VS, Kumar RSS, Sreeja KR (2021). Impact of environmental conditions on the levels of stress and breeding performance in Wistar rats: conventional environment versus environmentally controlled housing. T. Appl. Biol. Chem. J; 2(2):53-58. https://www.google.com/url?q=https%3A%2F%2Fdoi.org%2F10.52679%2Ftabcj.2021.0009&sa=D&sntz=1&usg=AFQjCNEKpT8RvaxFIrAlK-AVHdT9e9jbug

Harikrishnan VS. ?"What a cool facility!!! " - but how do the inhabitants feel??- Journal of Laboratory Animal Science, January-June 2020, Pp14-16. (Non-indexed Journal of LASA, India).

Mejo C Korah, SP Hima, Silpa Raj, Arya Anil, VS Harikrishnan, Lissy Krishnan. Pharmacokinetics and pharmacodynamics of avian egg-yolk derived pure anti-snake venom in healthy and disease animal-model. Journal of Pharmaceutical Sciences. Available online 20 February 2022. https://doi.org/10.1016/j.xphs.2022.02.008

Jayakumari, Nandini Ravikumar, Raji Sasikala Rajendran, Ashok Sivasailam, Sulfath Thottungal Parambil, Anand Chellappan Reghuvaran, Harikrishnan Vijayakumar Sreelatha, and Srinivas Gopala. "Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart." European Journal of Pharmacology (2021): 173918. https://doi.org/10.1016/j.ejphar.2021.173918 IF- 4.432

Raji, Sasikala Rajendran, Ravikumar Jayakumari Nandini, Sivasailam Ashok, Reghuvaran Chellappan Anand, Pillai Velayudhan Vivek, Jayakumar Karunakaran, Harikrishnan Vijayakumar Sreelatha, Shankarappa Manjunatha, and Srinivas Gopala. "Diminished substrate?mediated cardiac mitochondrial respiration and elevated autophagy in adult male offspring of gestational diabetic rats." IUBMB life (2021). https://doi.org/10.1002/iub.2447 IF- 3.885

Ramakrishnan R, Harikrishnan VS, Anil A, Arumugham S, Krishnan LK. Extracellular matrix?based combination scaffold for guided regeneration of large?area full?thickness rabbit burn wounds upon a single application. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2021 Nov 13. https://doi.org/10.1002/jbm.b.34965 IF- 3.368

Chandrababu, Krishnapriya, Harikrishnan Vijayakumar Sreelatha, Tara Sudhadevi, Arya Anil, Sabareeswaran Arumugam, and Lissy Kalliyana Krishnan; In vivo neural tissue engineering using adipose-derived mesenchymal stem cells and fibrin matrix; The Journal of Spinal Cord Medicine (2021): 1-15. https://doi.org/10.1080/10790268.2021.1930369 IF- 1.35

Ramakrishnan R, Venkiteswaran K, Sreelatha HV, Lekshman A, Arumugham S, KalliyanaKrishnan L. Assembly of skin substitute by cross-linking natural biomaterial on synthetic biodegradable porous mat for critical-size full-thickness burn wound regeneration. Biomed Mater. 2022 Feb 15. Epub ahead of print. PMID: 35168228. https://doi.org/10.1088/1748-605x/ac5573

Ramakrishnan R, Sreelatha HV, Anil A, Arumugham S, Varkey P, Senan M, Krishnan LK. Human-Derived Scaffold Components and Stem Cells Creating Immunocompatible Dermal Tissue Ensuing Regulated Nonfibrotic Cellular Phenotypes. ACS Biomaterials Science & Engineering. 2020 Apr 7;6(5):2740-56. https://doi.org/10.1021/acsbiomaterials.9b01961 IF- 4.749

Sudhadevi, Tara, Harikrishnan Vijayakumar Sreelatha, Easwer V. Hariharan, Samavedam Sandhyamani, and Lissy K. Krishnan. "Optimizing fibrin hydrogel toward effective neural progenitor cell delivery in spinal cord injury." Biomedical Materials (2021). PMID: 34736245 DOI: https://doi.org/10.1088/1748-605X/ac3680 IF- 3.715

Sherly, C.D., Rekha, M.R. and Harikrishnan, V.S., 2020. Cationised dextran and pullulan modified with diethyl aminoethyl methacrylate for gene delivery in cancer cells. Carbohydrate Polymers, p.116426. https://doi.org/10.1016/j.carbpol.2020.116426 IF- 9.381

Rajan RK, Chandran S, Sreelatha HV, John A, Parameswaran R. Pamidronate encapsulated electrospun polycaprolactone based composite scaffolds for osteoporotic bone defect repair. ACS Applied Bio Materials. 2020 Mar 6. https://doi.org/10.1021/acsabm.9b01077 IF- 3.25

Ajit A, Krishnan LK, Harikrishnan VS, Varkey P, Sabareeswaran A. Exploring Cadaver Skin for Standardization of Rabbit And Porcine Burn Models In Research. Annals of Burns and Fire Disasters. 2020 Dec 31;33(4):334. PMID: 33708025 PMCID: PMC7894838 IF- 0.83

Anand CR, Bhavya B, Jayakumar K, Harikrishnan VS, Gopala S. Inorganic nitrite alters mitochondrial dynamics without overt changes in cell death and mitochondrial respiration in cardiomyoblasts under hyperglycemia. Toxicology in Vitro. 2020 Nov 5:105048. https://doi.org/10.1016/j.tiv.2020.105048 IF- 2.903

Jose M, Sreelatha HV, James MV, Arumughan S, Thomas SV. Teratogenic effects of carbamazepine in mice. Annals of Indian Academy of Neurology. 2017 Apr;20(2):132. PMCID: PMC5470153 PMID: 28615898 https://dx.doi.org/10.4103%2Faian.AIAN_492_16 IF- 0.77

Mir Mahmoud Mortazavi R, Suresh Babu S., Harikrishnan V.S. and Annie John. A functional injectable hydrogel for nucleus pulposus regeneration in Lapine intervertebral disc defect model. European Journal of biomedical and pharmaceutical sciences, 2017, Volume 4, Issue 8, 587-602.

Venugopal, B., Fernandez, F., Harikrishnan, V.S. et al. Post implantation fate of adipogenic induced mesenchymal stem cells on Type I collagen scaffold in a rat model. J Mater Sci: Mater Med 28, 28 (2017). https://doi.org/10.1007/s10856-016-5838-7 IF- 3.57

Sunitha C, Harikrishnan VS, Annie J. Ovariectomised and long term induced (oli) rat model for preclinical assessment of osteoporotic therapeutics. Journal of Drug Delivery and Therapeutics. 2017 Mar 15;7(2):36-43. https://doi.org/10.22270/jddt.v7i2.1400

Chandran S, Babu S S, VS Harikrishnan, Varma HK, John A. Osteogenic efficacy of strontium hydroxyapatite micro-granules in osteoporotic rat model. Journal of biomaterials applications. 2016 Oct;31(4):499-509. https://doi.org/10.1177%2F0885328216647197 IF- 2.2558

Nair R, Harkrishnan VS, Jasmin J, Kalliyana K, Lissy KK (2016). Autologous circulating progenitor cells transplanted with hybrid scaffold accelerate diabetic wound healing in rabbit model. Current Tissue Engineering. 3. 137-146. http://dx.doi.org/10.2174/2211542005666160328200718 IF- 3.616

Jayakumari NR, Reghuvaran AC, Rajendran RS, Pillai VV, Karunakaran J, Sreelatha HV, Gopala S. Are nitric oxide-mediated protein modifications of functional significance in diabetic heart? ye'S,-NO?, wh `Y-NO?t?. Nitric Oxide. 2014 Dec 1; 43: 35-44. https://doi.org/10.1016/j.niox.2014.08.002 IF- 4.367

Ismael S, Purushothaman S, Harikrishnan VS, Nair RR. Ligand specific variation in cardiac response to stimulation of peroxisome proliferator-activated receptor-alpha in spontaneously hypertensive rat. Molecular and cellular biochemistry. 2015 Aug 1;406(1-2):173-82. https://doi.org/10.1007/s11010-015-2435-x IF- 3.396

Krishnan LK, Saroja JB, Rajalingam M, John V, Valappil MP, Sreelatha HV. Rabbit snake-bite model to assess safety and efficacy of anti viper chicken antibodies (IgY). American Journal of Clinical and Experimental Medicine. 2015;3(1):32-8.

Saraswathy A, Nazeer SS, Jeevan M, Nimi N, Arumugam S, Harikrishnan VS, Varma PH, Jayasree RS. Citrate coated iron oxide nanoparticles with enhanced relaxivity for in vivo magnetic resonance imaging of liver fibrosis. Colloids and Surfaces B: Biointerfaces. 2014 May 1; 117: 216-24. https://doi.org/10.1016/j.colsurfb.2014.02.034 IF- 3.997

Oloyo AK, Sofola OA, Anigbogu CN, Nair RR, Vijayakumar HS, Fernandez AC. Testosterone reduces vascular relaxation by altering cyclic adenosine monophosphate pathway and potassium channel activation in male Sprague Dawley rats fed a high-salt diet. Therapeutic advances in cardiovascular disease. 2013 Apr;7(2):75-85. https://doi.org/10.1177/1753944713479996

Venugopal B, Fernandez FB, Babu SS, Harikrishnan VS, Varma H, John A. Adipogenesis on biphasic calcium phosphate using rat adipose?derived mesenchymal stem cells: In vitro and in vivo. Journal of Biomedical Materials Research Part A. 2012 Jun;100(6):1427-37. https://doi.org/10.1002/jbm.a.34082 IF- 4.396

Mathai A, Neelima R, Harikrishnan VS, Radhakrishnan VV. Trehalose 6, 6?dimycolate (cord factor) induced pulmonary granuloma in naive and lymphopenic mice. Journal of Infectious Diseases and Immunity. 2012 Mar 31;4(3):29-35. https://doi.org/10.5897/JIDI2.003

Rose C, Neelima R, Mathai A, Harikrishnan VS, Radhakrishnan VV. Estimation of cord factor antigen of Mycobacterium tuberculosis in pleural fluid and its diagnostic significance. J. Infect. Dis. Immun.. 2012;4:36-40. https://doi.org/10.5897/JIDI12.009

Mathai A, Neelima R, Harikrishnan VS, Radhakrishnan VV. An immunoassay using cord factor antigen of Mycobacterium tuberculosis for the confirmatory diagnosis of intracranial tuberculoma. Journal of Infectious Diseases and Immunity. 2011 Apr 30;3(4):64-7. https://doi.org/10.5897/JIDI.9000006

Sankar V, Nair RR, Harikrishnan VS, Fernandez AC, Kumar CS, Madhavachandran V. Cardoguard, an Ayurvedic antihypertensive formulation, prevents cardiac remodeling in spontaneously hypertensive rats by inhibition of ERK and PKC? signaling pathways. Canadian journal of physiology and pharmacology. 2012 May;90(5):627-35. https://doi.org/10.1139/y2012-047 IF-1.946

Oloyo AK, Harikrishnan VS, Sofola OA, Anigbogu CN. Testosterone Enhances the Early Onset and Promotes the Increase in Magnitude of Salt-Induced Hypertension in Male Sprague-Dawley Rats. African Journal of Biomedical Research. 2011;14(1):27-34. https://www.ajol.info/index.php/ajbr/article/view/95227 IF- 0.2

Oloyo, Ahmed Kolade, Olusoga Adekunle Sofola, Chikodi N. Anigbogu, Renuka R. Nair, and Vijayakumar Harikrishnan. "Orchidectomy Reduced Abdominal Aorta Relaxation Response to Androgens in Sprague?Dawley Rats Fed a High Salt Diet." Federation of American Societies for Experimental Biology (2011): 640-19. https://doi.org/10.1096/fasebj.25.1_supplement.640.19 IF- 4.966

Oloyo AK, Sofola OA, Nair RR, Harikrishnan VS, Fernandez AC. Testosterone relaxes abdominal aorta in male Sprague?Dawley rats by opening potassium (K+) channel and blockade of calcium (Ca2+) channel. Pathophysiology. 2011 Jun 1;18(3):247-53. https://doi.org/10.1016/j.pathophys.2011.02.004 IF- 2.15

Kaur S, Harikrishnan VS, Radhakrishnan S, Shenoy SJ, Uruno A, Sugawara A. Transfection of endothelial nitric oxide synthase gene improves angiogenic efficacy of endothelial progenitor cells in rabbits with hindlimb ischemia. J Clin Exp Cardiol. 2011;2:140. http://dx.doi.org/10.4172/2155-9880.1000140 IF- 0.758

Purushothaman S, Nair RR, Harikrishnan VS, Fernandez AC. Temporal relation of cardiac hypertrophy, oxidative stress, and fatty acid metabolism in spontaneously hypertensive rat. Molecular and cellular biochemistry. 2011 May 1;351(1-2):59-64. https://doi.org/10.1007/s11010-011-0711-y IF- 3.396

Anilkumar TV, Harikrishnan VS, Vineetha VP, Thulaseedharan NK, Sreeja KR, Deepa R, Fernandez AC. Immunohistochemistry and confocal microscopy of rat mammary gland scirrhous carcinoma. Indian Journal of Veterinary Pathology. 2011;35(1):108-10. https://www.indianjournals.com/ijor.aspx?target=ijor:ijvp&volume=35&issue=1&article=033

Kartha C, Kaur S, Harikrishnan V, Shenoy S, Jayakumar K, Kumar S. Transfection with endothelial nitric oxide synthase (eNOS) improves functions of circulating endothelial progenitor cells from coronary patients. Fundamental and Clinical Pharmacology. 2010 Feb;24(1). IF 2.19

Oloyo, Ahmed Kolade, Olusoga A. Sofola, Renuka R. Nair, V. S. Harikrishnan, and Adelaide C. Fernandez. "Effect of orchidectomy on vascular relaxation responses to cAMP and K channel activation in male Sprague Dawley rats fed a high salt diet." Journal of physiological sciences. 59; 452-452 (2009). IF- 0.37

Julie B, Harikrishnan VS, Baby PG. Mixed haemoprotozoan infection in calves. Indian Veterinary journal. 2005;82(5):543-4.

Harikrishnan VS. Ethical issues in animal experimentation. P.V. Mohanan Ed. In: Biomedical Product and Materials Evaluation 2022 Jan 1 (pp. 355-372). Woodhead Publishing. https://doi.org/10.1016/B978-0-12-823966-7.00004-9

Harikrishnan, V. S. "Laboratory Animal Behaviour and Its Applications in Biomedical Research." In Essentials of Laboratory Animal Science: Principles and Practices, pp. 479-495. Springer, Singapore, 2021. https://doi.org/10.1007/978-981-16-0987-9_19

Harikrishnan, V. S. "Anaesthesia, Analgesia and Euthanasia of Laboratory Rodents and Rabbits: A Practical Approach." In Essentials of Laboratory Animal Science: Principles and Practices, pp. 541-562. Springer, Singapore, 2021. https://doi.org/10.1007/978-981-16-0987-9_22

Balu Venugopal, B. F. Francis, Susan Mani, V. S. Harikrishnan, H. K. Varma, and Annie John. ?Microscopy Applied to Materials Sciences and Life Sciences? Assessing Adipose Tissue Engineering In Vitro and In Vivo: A Microscopic Approach, CRC Press, 2018.