Central Analytical Facility

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Central Analytical Facility (CAF) is equipped with the state of the art instruments for the analysis of materials, especially, polymers and nanomaterials. CAF provides testing services strictly adhering to the quality management policy of the institute. Wherever applicable, the tests are done as per ISO standards/other international standard guidelines. Many of the instruments in this facility were procured with a grant from the Department of Science & Technology, Govt. of India, under the project named “Facility for nano/microparticle based biomaterials for Advanced Drug Delivery Devices” (FADDS) vide DST approval No.VI-D&P/241/07-08/TDT dated 31.03.2008. The facility is open to the internal and external customers on a payment basis. For test charges and inquiries, kindly contact customer service cell (csc@sctimst.ac.in or (0471) 2520307 or 2520309).

List of analytical instruments available at CAF, their specifications and some of their applications are given below:
1. UV-Visible Spectrophotometer, Cary 100 Bio, Varian Inc., USA.
Wavelength range: 190 to 900 nm.
Double beam
Qualitative analysis and quantitative determination of UV/Vis active analytes, namely,organic compounds, biological macromolecules, etc.
UV-Visible Spectrophotometer
2. Fourier Transform Infra Red Spectrometer, Nicolet 5700, ThermoFisher Scientific, USA.
Spectral range: 4000 cm-1 - 350 cm-1.
Resolution: 0.4 cm-1
Diamond ATR
Determination of the functional groups present in compounds.
Determination of molecular structure.
Follow the course of chemical reactions.
Fourier Transform Infra Red Spectrometer, Nicolet 5700
3. Analytical Gel Permeation Chromatograph / High Performance Liquid Chromatograph, Waters, USA.
Detectors: UV (Waters 2487), RI (Waters 2414), ELS (Waters 2424).
Column: C18
Purity assay
Analytical separation
Molecular weight determination using Gel Permeation Chromatography
Analytical Gel Permeation Chromatograph
4. High Performance Liquid Chromatograph, LC-2010A HT, Shimadzu, Japan.
Detector: UV
Autosampler: up to 210 samples at one go
Column: C18
Analytical separation of individual chemical compounds from complex mixtures
Identification and quantification of compounds
High Performance Liquid Chromatograph
5. Micromass Quattro Micro LC/MS/MS system, Waters, USA.
ESI and APCI probes
Waters 2695 Alliance separation Module
Mass range: 20 to 2040 m/z
Trace level analysis of known analytes
Drug metabolite and pharmacokinetics studiess
Structural elucidation
Micromass Quattro Micro
6. Headspace-Gas Chromatograph, Clarus 580, Perkin Elmer, USA.
Flame Ionization Detector
Analysis of volatile residues.
Analysis of ethylene oxide residue.
Headspace-Gas Chromatograph Clarus 580
7. Differential Scanning Calorimeter, Q100, TA Instruments, USA.
Scan temperature range: -90 to 600oC (nitrogen atmosphere).
Modulated DSC option for understanding complex thermal curves and to increase the sensitivity of weak transitions.
Determination of the glass transition temperatures and enthalpies of fusion and crystallisation of materials.
Determination of specific heat, oxidative thermal stability, rate and degree of cure, and purity of materials.
Differential Scanning Calorimeter Q100
8. Differential Scanning Calorimeter, Q20, TA Instruments, USA.
Equipped with a refrigerated cooling system
Scan temperature range: -900C to 5500C (nitrogen atmosphere).
Determination of heat flow associated with phase transitions / reactions such as melting, crystallization, glass transition, curing, sorption, etc.
Determination of crystallinity, degree of curing, heat capacity, crystalline impurities, etc.
Differential Scanning Calorimeter Q20
9. Simultaneous DTA-TGA (Differential thermal analysis and Thermogravimetric analysis), SDT Q 600, TA Instruments, USA.
Temperature range: Room temperature to 15000C (nitrogen atmosphere).
Measures both weight changes & enthalpy changes associated with transitions and reactions in a material as a function of temperature (or time) under a controlled atmosphere
Thermal stability of the materials and their compositional analysis.
Differentiates endothermic and exothermic events, which are not associated weight change (e.g. melting and crystallization) from those which involve weight changes (e.g. degradation).
Simultaneous DTA-TGA
10. Texture Analyser, TA.XT Plus, Stable Micro Systems, UK.
Force range: +/-50Kg
Force resolution: 0.1gm
Speed range: 0.01-20mm/sec
Mechanical testing of films/tissues (Compression and tension)
Tests fracturability, mucoadhesiveness, gel strength, peel strength, burst strength, etc.
Consistency measurements of emulsions, creams and gels, adhesiveness of patches, etc.
Texture Analyser TA XT Plus
11. Confocal Raman Microscope with Atomic Force Microscope Accessory, Alpha300 RA, WITec GmbH, Germany.
Excitation Laser 1. Frequency doubled Nd:YAG dye laser [max power output 40 mW at 532 nm]
Excitation Laser 2. High Power Single Frequency Diode Laser [300 mW output power at 785 nm]
Contact mode and AC mode (tapping) in AFM mode
Signal detected: Topography
Vertical resolution: <0.3nm
Lateral resolution: 5nm (50A)
Scan range limits: 200micrometer x 200micrometer
Mapping: Yes
Determination of chemical structure of molecules by Micro Raman Spectroscopy
Confocal Raman Spectroscopy imaging (hyperspectral image generation with information of complete Raman Spectrum at pixel level).
Chemical mapping of distribution of components in a mixture by Raman imaging and depth profiling, e.g., drug in excipients/tablets, drug eluting stent coatings, live cell imaging etc.
Atomic Force Microscopy – Acquisition of AFM contact and AFM AC (non-contact) mode images. Surface topography on the nanometer scale. Lateral resolution up to 1nm. Depth resolution < 0.3 nm.
Confocal Raman Microscope with Atomic Force Microscope Accessory Alpha 300
12. Fluorescent Microscope, DMI3000B, Leica, Germany.
Inverted microscope; Light source: EL6000; Digital camera: DFC 420C; Filter cubes: A, I3, N2.1 & GFP + bright field; Software: Leica Application Suite V3.8
Objectives: 5x N Plan NA = 0.12 WD = 14mm; 10 x HI Plan NA = 0.22 WD = 7.8mm; 20 x N Plan NA = 0.35 WD = 6.9mm; 40 x N Plan NA = 0.55 WD = 3.3-1.9; 63 x PL Fluotar NA = 0.7 WD = 2.6mm
Quick and easy screening of fluorescent samples
Live cell imaging.
Fluorescent Microscope DMI3000B
13. Multifunctional Microplate Reader, Infinite M200, TECAN, Austria
Monochromator based equipment that supports 6-384 well microplate formats.
Fluorescence Intensity measurement: Top & Bottom
Fluorescence Time Resolved measurements
Absorbance range: 230nm-1000nm
Fluorescence scan – Excitation: 230-850nm; Emission: 280-850nm
Fluorescence Intensity and Absorbance measurements with microplates
Fluorescence and Absorbance scan with microplates
Microplate Reader Infinite M200
14. Electrochemical Quartz Crystal Nanobalance, EQCN-700, ELCHEMA, USA.
Mass change ranges: +/- 100µg to +/- 100ng
Resolution: 0.01ng
Piezoelectric nanogravimetry for the measurement of mass changes on an electrode surface in the range of ng/cm2
Detection of atomic monolayers forming on the surfaces.
Study of interfacial processes occurring at or near the electrode surface such as surface adsorption or desorption.
Study of protein interfacial behaviours and adsorption behaviour of biologically active molecules
15. Luminescent Image Analyser, LAS 4000, Fuji Film, Japan.
Filters available – 460nm blue, 520nm green, 630nm red, 710nm IR, 312 & 365 nm UV and white light.
Multipurpose CCD camera system for sensitive and quantitative imaging of membranes, gels, small animals, colony counting, etc.
LAS 4000
16. Rapid Visco Analyser, RVA Starch Master 2, Newport Scientific, Australia.
Viscosity range: 40-14000cP at 80rpm, 20-8000 at 160rpm
Viscosity accuracy: ±3%
Speed range: 20-1000rpm
Temperature range: ambient to 990C
For the determination of solution viscosity of biopolymers
Stirring viscometer with ramped temperature and changeable shear capability for the testing of viscous properties of starch, alginate, chitosan, hydrocolloids, proteins, etc.
Rapid Visco Analyser
17. Environmental chamber (CM Enviro Systems, 225 L), India.
Temperature range: 2-900C
Humidity range: 20-90% RH
Light: On/Off
Aging/stability studies in specified environmental conditions
Accelerated ageing/stability studies
Shelf life estimation
CM Enviro Systems